Publications

EU Research (AUT21/P37) spoke to Dr Aditee Mitra and Dr Xabier Irigoien about how the MSCA-ITN MixITiN has been bringing marine ecology into the 21st century, work which holds important implications for ocean health and policies in this UN Ocean Decade.

Selected publications on mixoplankton; MixITiN staff indicated in bold

Anestis K, Kohli GS, Wohlrab S, Varga E, Larsen TO, Hansen PJ, John U (2021) Polyketide synthase genes and molecular trade-offs in the ichthyotoxic species Prymnesium parvum. Science of the Total Environment https://doi.org/10.1016/j.scitotenv.2021.148878

Glibert PM, Beusen AH, Bouwman AF, Burkholder JM, Flynn KJ, Heil CA, Li M, Lin CHM, Madden CJ, Mitra A, Nardin W (2021) 12 Multifaceted climatic change and nutrient effects on harmful algae require multifaceted models. pp:473-518. In: Climate Change and Marine and Freshwater Toxins Editors: Botana LM, Louzao MC, Vilariño N. DeGruyter Publishers https://doi.org/10.1515/9783110625738-012

Hughes EA, Maselli M, Sørensen H, Hansen PJ (2021) Metabolic reliance on photosynthesis depends on both irradiance and prey availability in the mixotrophic ciliate, Strombidium cf. basimorphum.  Frontiers in Microbiology 12:1577. doi: 10.3389/fmicb.2021.642600

Leles SG, Bruggeman J, Polimene L, Blackford J, Flynn KJ, Mitra A (2021) Differences in physiology explain succession of mixoplankton functional types and affect carbon fluxes in temperate seas. Progress in Oceanography 190:102481 https://doi.org/10.1016/j.pocean.2020.102481

Mansour JS, Anestis K (2021) Eco-Evolutionary Perspectives on Mixoplankton Frontiers in Marine Science  https://doi.org/10.3389/fmars.2021.666160

Mansour J, Not F (2021) Isolation of clean single-cell samples for physiological or molecular experiments (Radiolaria, Acantharia) V.2. protocols.io. dx.doi.org/10.17504/protocols.io.bqvrmw56

Mansour J, Anestis K, Not F, John U (2021) cDNA library preparation from total RNA extracts of Single-cell marine protists (e.g., Acantharia, Strombidium basimorphum, and Prymnesium parvum) for transcriptome sequencing V.2. protocols.io. dx.doi.org/10.17504/protocols.io.brw3m7gn

Mansour J, Anestis K, Not F, John U (2021) Single-cell total RNA extraction from marine protists (e.g., Acantharia, Strombidium cf basimorphum, and Prymnesium parvum) V.2. protocols.io. dx.doi.org/10.17504/protocols.io.bvhyn37w

Mansour JS, Norlin A, Llopis Monferrer N, L’Helguen S, Not F (2021) Carbon and nitrogen content to biovolume relationships for marine protist of the Rhizaria lineage (Radiolaria and Phaeodaria). Limnology and Oceanography 66:1703-1717

Maselli M, Anestis K, Klemm K, Hansen PJ, John U (2021) Retention of prey genetic material by the kleptoplastidic ciliate Strombidium cf. basimorphum. Frontiers in Microbiology 10.3389/fmicb.2021.694508

Mitra A, Flynn KJ (2021) HABs and the Mixoplankton Paradigm. Harmful Algae News 67, 4-6  http://doi.org/10.5281/zenodo.5109703

Romano F, Pitta P (2021) Relationships of pelagic ciliates with the microbial food web components at a coastal station in the oligotrophic Eastern Mediterranean Sea: temporal and vertical variability. Journal of Plankton Research 1:21. https://doi.org/10.1093/plankt/fbab053

Romano F, Symiakaki K, Pitta P (2021) Temporal Variability of Planktonic Ciliates in a Coastal Oligotrophic Environment: Mixotrophy, Size Classes and Vertical Distribution Frontiers in Marine Science https://doi.org/10.3389/fmars.2021.641589

Royer C, Borges AV, Lapeyra Martin J, Gypens N (2021) Drivers of the variability of dimethylsulfonioproprionate (DMSP) and dimethylsulfoxide (DMSO) in the Southern North Sea. Continental Shelf Research 216 : 104360 https://doi.org/10.1016/j.csr.2021.104360

Anschütz AA, Flynn KJ (2020) Niche separation between different functional types of mixoplankton: results from NPZ-style N-based model simulations. Marine Biology 167:3 https://doi.org/10.1007/s00227-019-3612-3

Ferreira GD, Calbet A (2020) Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans. Scientific Reports 10:3899 https://doi.org/10.1038/s41598-020-60764-2

Flynn KJ, Skibinski DOF (2020) Exploring evolution of maximum growth rates in plankton. Journal of Plankton Research 42:497–513 doi:10.1093/plankt/fbaa038

Maselli M, Altenburger A, Stoecker DK, Hansen PJ (2020) Ecophysiological traits of mixotrophic Strombidium spp. Journal of Plankton Research 42: 485–496 https://doi.org/10.1038/s41598-020-69174-w

Schneider LK, Flynn KJ, Herman PMJ, Troost TA, Stolte W (2020) Exploring the Trophic Spectrum: Placing Mixoplankton Into Marine Protist Communities of the Southern North Sea. Frontiers in Marine Science 7:586915 https://doi.org/10.3389/fmars.2020.586915

Traboni C, Calbet A, Saiz E (2020) Effects of prey trophic mode on the gross-growth efficiency of marine copepods: the case of mixoplankton. Scientific Reports 10:12259 https://doi.org/10.1038/s41598-020-69174-w

Borges AV, Royer C, Lapeyra Martin J, Champenois W, Gypens N (2019) Response of Marine Methane Dissolved Concentrations and Emissions in the Southern North Sea to the European 2018 Heatwave. Continental Shelf Research 190:104004 https://doi.org/10.1016/j.csr.2019.104004

Flynn KJ, Mitra A, Glibert PM, Burkholder J-AM (2018) Mixotrophy in Harmful Algal Blooms: By Whom, on Whom, When, Why, and What Next. In: Glibert P., Berdalet E., Burford M., Pitcher G., Zhou M. (eds) Global Ecology and Oceanography of Harmful Algal Blooms. Ecological Studies (Analysis and Synthesis), vol 232. Springer, Cham https://doi.org/10.1007/978-3-319-70069-4_7

Flynn KJ, Mitra A, Anestis K, Anschütz AA, Calbet A, Ferreira GD, Gypens N, Hansen PJ, John U, Martin JL, Mansour J, Maselli M, Medić N, Norlin A, Not F, Pitta P, Romano F, Saiz E, Schneider L, Stolte W, Traboni C (2019) Mixotrophic protists and a new paradigm for marine ecology: where does plankton research go now? Journal of Plankton Research https://doi.org/10.1093/plankt/fbz026

Glibert P, Mitra A, Flynn K, Hansen P, Jeong H and Stoecker D (2019) Plants Are Not Animals and Animals Are Not Plants, Right? Wrong! Tiny Creatures in the Ocean Can Be Both at Once! Front. Young Minds 7: 48. https://doi.org/10.3389/frym.2019.00048

Leles SG, Mitra A, Flynn KJ, Tillmann U, Stoecker D, Jeong HJ, Burkholder J, Hansen PJ, Caron DA, Glibert PM, Hallegraeff G (2019) Sampling bias misrepresents the biogeographical significance of constitutive mixotrophs across global oceans. Glob. Ecol. Biogeogr. 28: 418-428 https://doi.org/10.1111/geb.12853

Svenssen, DK, Binzer, SB, Medić N, Hansen PJ, Larsen TO, & Varga E (2019) Development of an indirect quantitation method to assess ichthyotoxic b-type prymnesins from Prymnesium parvum. Toxins 11:251 https://doi.org/10.3390/toxins11050251

Leles SG, Polimene L, Bruggeman J, Blackford J, Ciavatta S, Mitra A, Flynn KJ (2018) Modelling mixotrophic functional diversity and implications for ecosystem function. J. Plankton Res., 40: 627-642 https://doi.org/10.1093/plankt/fby044

Lin C-H, Flynn KJ, Mitra A, Glibert PM (2018) Simulating Effects of Variable Stoichiometry and Temperature on Mixotrophy in the Harmful Dinoflagellate Karlodinium veneficum. Front. Mar. Sci. 5: 320 doi:10.3389/fmars.2018.00320

Ghyoot C, Flynn KJ, Mitra A, Lancelot C, Gypens N (2017) Modeling plankton mixotrophy: a mechanistic model consistent with the Shuter-type biochemical approach. Front. Ecol. Evol. https://doi.org/10.3389/fevo.2017.00078

Ghyoot C, Lancelot C, Flynn KJ, Mitra A, Gypens N (2017) Introducing mixotrophy into a biogeochemical model describing an eutrophied coastal ecosystem: The Southern North Sea. Prog. Oceanogr. 157: 1-11 http://dx.doi.org/10.1016/j.pocean.2017.08.002

Leles SG, Mitra A, Flynn KJ, Stoecker DK, Hansen PJ, Calbet A, McManus GB, Sanders RW, Caron DA, Not F, Hallegraeff GM, Pitta P, Raven JA, Johnson MD, Glibert PM, Våge S. (2017) Oceanic protists with different forms of acquired phototrophy display contrasting biogeographies and abundance. Proc. Roy. Soc. B. doi: 10.1098/rspb.2017.0664

Mitra A, Flynn KJ, Tillmann U, Raven JA, Caron D, Stoecker DK, Not F, Hansen PJ, Hallegraeff G, Sanders R, Wilken S, McManus G, Johnson M, Pitta P, Våge S, Berge T, Calbet A, Thingstad F, Jeong HJ, Burkholder J, Glibert PM, Granéli E, Lundgren V (2016) Defining planktonic protist functional groups on mechanisms for energy and nutrient acquisition; incorporation of diverse mixotrophic strategies. Protist 167: 106-120 https://doi.org/10.1016/j.protis.2016.01.003

Lundgren VM, Glibert PM, Granéli E, Vidyarathna NK, Fiori E, Ou L, Flynn KJ, Mitra A, Stoecker DK, Hansen PJ (2016) Metabolic and physiological changes in Prymnesium parvum when grown under, and grazing on prey of, variable nitrogen : phosphorus stoichiometry. Harmful Algae 55: 1-12 https://doi.org/10.1016/j.hal.2016.01.002

Ghyoot C, Gypens N, Flynn KJ, Lancelot C (2015). Modelling alkaline phosphatase activity in microalgae under orthophosphate limitation: the case of Phaeocystis globosa. J. Plankton Res. 37: 869-885. doi:10.1093/plankt/fbv062

Mitra A, Flynn KJ, Burkholder JM, Berge T, Calbet A, Raven JA, Granéli E, Glibert PM, Hansen PJ, Stoecker DK, Thingstad F, Tillmann U, Våge S, Wilken S, Zubkov MV (2014). The role of mixotrophic protists in the biological carbon pump. Biogeosciences 11: 995-1005 doi:10.5194/bg-11-995-2014

Flynn KJ, Hansen PJ (2013) Cutting the canopy to cefeat the “selfish gene”; conflicting selection pressures for the integration of phototrophy in mixotrophic protists. Protist 164: 811-823 https://doi.org/10.1016/j.protis.2013.09.002

Flynn KJ, Stoecker DK, Mitra A, Raven JA, Glibert PM, Hansen PJ, Granéli E, Burkholder JM (2013) Misuse of the phytoplankton-zooplankton dichotomy: the need to assign organisms as mixotrophs within plankton functional types Journal of Plankton Research 35, 3-11 doi:10.1093/plankt/fbs062.

Mitra A, Flynn KJ (2010) Modelling mixotrophy in Harmful Algal Blooms; more or less the sum of the parts? J. Mar. Sys. 83: 158-169 https://doi.org/10.1016/j.jmarsys.2010.04.006

Flynn KJ, Mitra A (2009) Building the “perfect beast”: modelling mixotrophic plankton. J. Plankton Res. 31: 965-992 https://doi.org/10.1093/plankt/fbp044